Astigmatic multifocus microscopy enables deep 3D super-resolved imaging
نویسندگان
چکیده
منابع مشابه
Multifocus structured illumination microscopy for fast volumetric super-resolution imaging.
We here report for the first time the synergistic implementation of structured illumination microscopy (SIM) and multifocus microscopy (MFM). This imaging modality is designed to alleviate the problem of insufficient volumetric acquisition speed in super-resolution biological imaging. SIM is a wide-field super-resolution technique that allows imaging with visible light beyond the classical diff...
متن کاملSuper - resolved fluorescence microscopy
THE ROYAL SWEDISH ACADEMY OF SCIENCES has as its aim to promote the sciences and strengthen their influence in society.
متن کاملSuper Temporal-Resolved Microscopy (STReM).
Super-resolution microscopy typically achieves high spatial resolution, but the temporal resolution remains low. We report super temporal-resolved microscopy (STReM) to improve the temporal resolution of 2D super-resolution microscopy by a factor of 20 compared to that of the traditional camera-limited frame rate. This is achieved by rotating a phase mask in the Fourier plane during data acquis...
متن کاملSuper-resolved Ptychographic Imaging
Ptychography is a form of Coherent Diffractive Imaging (CDI) in which a localized illumination function (or probe) is sent to a grid of positions on a sample and a detector records a diffraction pattern at each position. Setting the pitch of the position grid smaller than the size of the probe ensures that most points on the sample are illuminated multiple times, whilst structure in the probe e...
متن کاملFluorescence microscopy with super-resolved optical sections.
The fluorescence microscope, especially its confocal variant, has become a standard tool in cell biology research for delivering 3D-images of intact cells. However, the resolution of any standard optical microscope is at least 3 times poorer along the axis of the lens that in its focal plane. Here, we review principles and applications of an emerging family of fluorescence microscopes, such as ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biomedical Optics Express
سال: 2016
ISSN: 2156-7085,2156-7085
DOI: 10.1364/boe.7.002163